AccuRuler Prestained Protein Ladder

A pre-stained protein molecular weight marker containing 10 proteins ranging from 10 to 180 kDa, formulated for protein electrophoresis and Western blot applications.

Technical Specifications:
- Size range: 10-180 kDa
- Number of bands: 10
- Reference bands: Red (~75 kDa), Green (~25 kDa)
- Volume: 2 x 250 µl
- Recommended loading: 3-5 µl per well

Buffer Composition:
- 20 mM Trisphosphate (pH 7.5 at 25°C)
- 2% SDS
- 1 mM reducing reagent
- 3.6 M Urea
- Protein concentration: 0.1-0.4 mg/ml each
- Includes stabilizers

Research Applications:
- Protein size determination
- PAGE protein separation
- Western blot transfer monitoring
- Molecular weight estimation
- Protein migration tracking

Protocol Features:
- Ready-to-use format
- No heating required
- No dilution needed
- No additional reducing agents required
- Room temperature handling

Storage:
- Store at -20°C
- Avoid repeated freeze-thaw cycles

Keywords: protein ladder, molecular weight marker, Western blot standard, PAGE marker, protein size standard