1KB Plus DNA Ladder is a proprietary mixture of several plasmids digested to completion with specific restriction enzymes to produce a ladder with 13 fragments ranging from 100bp - 10.0Kb, producing the most complete and convenient ladder in the 1Kb DNA ladder family.  The intensity of the some bands has been increased to yield internal reference indicators.  6µl will yield at least of 30ng DNA in any single DNA band.