Tsg DNA Polymerase

A thermostable DNA polymerase isolated from Thermus sp., engineered for PCR and primer extension applications in molecular biology research.

Technical Specifications:
- Thermal stability: 3-hour half-life at 95°C
- Error rate: 15x10^6 during DNA synthesis
- Terminal transferase activity: Adds single 3' A overhang
- Unit definition: One unit incorporates 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C
- Compatible with standard PCR protocols

Enzyme Properties:
- Isolated from Thermus sp.
- Thermostable at PCR temperatures
- Template-dependent DNA synthesis
- 3' A-overhang generation
- Active across standard PCR conditions

Research Applications:
- Standard PCR protocols
- High-throughput PCR procedures
- Molecular cloning projects
- DNA amplification studies
- TA cloning applications

Keywords: thermostable DNA polymerase, PCR enzyme, molecular biology reagents, DNA amplification, Thermus sp. polymerase, cloning enzyme