Tsg DNA Polymerase is a thermostable DNA polymerase isolated from a strain of Thermus sp., and designed for primer extension reactions and PCR. Tsg has a half-life of 3 hours at 95oC, which means it is more stable than other DNA polymerases. It is also a high fidelity polymerase, with an error frequency of 15x106 during DNA synthesis. DNA sequencing with Tsg DNA Polymerase produces uniform band intensities and low background. Tsg has independent terminal transferase activity, which results in the addition of a single adenosine (A) at the 3\' end of the extension product, thereby allowing the amplification product to be cloned in T-vectors.SPECIAL FEATURESExcellent yieldsAlternative for Taq PolymeraseGreater stabilityBest valueAPPLICATIONSGeneral PCR reactionsHigh-throughput PCRMolecular cloningComparison of an amplified 1.3kb mouse gene fragment using Tsg and Taq DNA Polymerases.Lane M: 1Kb DNA ladderLanes 1 and 2: Taq DNA polymerase from Company RLanes 3 and 4: Taq DNA polymerase from Company QLanes 5 and 6: Lamda Biotech\'s Tsg DNA Polymerase