The SafeStain Super-PCR Kit contains the SafeStain 2X PCR Master Mix and the  PreSafeStained 1Kb DNA Ladder. SafeStain is a non-carcinogenic green florescent dye for staining the DNA fragments directly in the PCR mix and the DNA ladder. Taq Plus DNA polymerase is used in the 2X PCR master mix with dNTP, Mg2+ and PCR reaction buffer optimally formulated for efficient amplification of DNA templates by PCR. Users only need to add template, primers for PCR, which greatly saves time and reduces contamination due to less pipetting steps needed for PCR set-up. When running the agarose gel, two non-toxic inert  dyes, blue and yellow, can viewed on the gel. The blue dye migrates at the position of a 4kb and the yellow dye at about 10bp of DNA fragment. The Pre-SafeStained 1Kb DNA Ladder contains 13  DNA fragments ranging from 100bp to 10Kb.