qPCR 2X MasterMix Universal for SYBR Green Assay
qPCR 2X MasterMix Universal for SYBR Green Assay
A pre-formulated master mix designed for real-time PCR applications using DNA-binding fluorescent dye chemistry. Contains modified DNA polymerase optimized for quantitative PCR analysis.
Technical Specifications:
- Format: 2X concentrated
- Detection: DNA-binding fluorescent dye
- Compatible with standard qPCR instruments
- Ready-to-use formulation
- Multiple size options available
Master Mix Components:
- Modified DNA polymerase
- Optimized reaction buffer
- DNA-binding fluorescent dye
- dNTPs
- PCR enhancers
Research Applications:
- Gene expression analysis
- DNA quantification
- Copy number determination
- miRNA studies
- Pathway analysis
- Validation studies
Protocol Features:
- Standard real-time PCR conditions
- Direct use without modifications
- Room temperature setup
- Minimal primer-dimer formation
- Compatible with various primer designs
Keywords: real-time PCR, qPCR master mix, gene expression analysis, molecular biology reagents