Proteinase K Solution

A broad-spectrum serine protease solution designed for protein degradation in molecular biology applications. This enzyme maintains activity across diverse buffer conditions and is resistant to multiple inhibitors.

Technical Specifications:
- Format: Ready-to-use solution
- Volume: 3 ml
- pH range: 4.0-12.5
- Optimal pH: 6.5-9.5
- Temperature optimum: 65°C
- Activity ratio: 12:1 (65°C vs 25°C)

Enzyme Properties:
- Resistant to metal ions
- Resistant to EDTA
- Resistant to sulfhydryl reagents
- Resistant to trypsin inhibitors
- Resistant to chymotrypsin inhibitors
- Enhanced by SDS and urea

Working Parameters:
- Recommended temperature: 55°C
- Maintains activity after partial autolysis
- Stable in various buffer conditions
- Compatible with denaturing agents
- Active against native proteins

Research Applications:
- Nucleic acid preparation
- Protein degradation studies
- Sample preparation protocols
- DNA/RNA isolation
- Tissue dissociation

Keywords: protease enzyme, protein degradation, molecular biology reagents, sample preparation enzyme