Pfu DNA Polymerase

A high-fidelity thermostable DNA polymerase isolated from Pyrococcus furiosus, produced in E. coli through recombinant expression. This enzyme is engineered for PCR applications requiring enhanced accuracy in DNA synthesis.

Enzyme Properties:
- Template-dependent 5'→3' DNA polymerization
- 3'→5' exonuclease proofreading activity
- No 5'→3' exonuclease activity
- Generates blunt-ended PCR products
- Thermostable at PCR temperatures

Technical Specifications:
- Source organism: Pyrococcus furiosus
- Expression system: E. coli
- Proofreading capability: Yes
- End modification: Blunt ends
- Temperature range: Compatible with standard PCR conditions

Research Applications:
- High-fidelity DNA amplification
- Cloning applications
- Site-directed mutagenesis
- Genomic DNA analysis
- Gene expression studies
- Molecular typing protocols

Keywords: high-fidelity PCR enzyme, proofreading DNA polymerase, molecular cloning reagents, DNA amplification, thermostable polymerase