Pfu 2X PCR Master Mix

A pre-formulated PCR master mix containing Pfu DNA polymerase with proofreading capability, designed for high-fidelity DNA amplification applications. Includes dual-color tracking dyes for visual monitoring of electrophoresis.

Technical Specifications:
- Format: 2X concentrated
- Enzyme: Pfu DNA polymerase
- End modification: Blunt ends
- Tracking dyes: Blue and yellow indicators
- Direct gel loading capability

Master Mix Components:
- Pfu DNA polymerase
- Optimized reaction buffer
- dNTPs
- Tracking dyes
- Stabilizers

Research Applications:
- High-fidelity DNA amplification
- Molecular cloning protocols
- Site-directed mutagenesis
- Gene expression studies
- DNA library construction
- Template generation for sequencing

Protocol Features:
- Simplified reaction setup
- Template and primer addition only
- Visual tracking during setup
- Direct gel loading capability
- Room temperature assembly

Keywords: Pfu polymerase, high-fidelity PCR, master mix, molecular biology reagents, PCR optimization