The One-Step TaqProbe qRT-PCR Kit is a complete quantitative RT-PCR kit for TaqMan assay in one step. It contains all reagents necessary for both Reverse Transcription (RT) and TaqMan Probe based qPCR amplification to occur in a single qPCR reaction tube. The One-Step TaqProbe qRT-PCR Kit is an integration of two key processes: the RT and the real-time PCR. With our innovative RT Master Mix, stabilizers and enhancers, and the hot-start qPCR enzyme, it enables a seamless coupling of two separate steps into one real-time “single step” process. It is very easy and useful for gene expression study and other practical applications. This kit works well on most of the real-time PCR machines.Kit ContentsTaqProbe qPCR MasterMix: 1250 µlqRT-PCR Enzyme Mix: 40 µlROX dye solution:: 15 µlNuclease-free H2O: 1000 µlStorage: Stored at -20° C