One-Step TaqProbe qRT-PCR Kit

A single-tube system designed for combined reverse transcription and probe-based real-time PCR analysis. Integrates RT and qPCR steps in a unified reaction for RNA quantification applications.

Technical Specifications:
Kit Components:
- TaqProbe qPCR MasterMix: 1250 µl
- qRT-PCR Enzyme Mix: 40 µl
- ROX dye solution: 15 µl
- Nuclease-free H₂O: 1000 µl
- Storage temperature: -20°C

System Features:
- Single-tube reaction format
- Hot-start PCR capability
- Integrated RT step
- ROX reference option
- Probe-based detection

Research Applications:
- Gene expression analysis
- RNA quantification
- Pathogen detection
- miRNA studies
- Viral load testing
- Transcript analysis

Protocol Features:
- Combined RT-PCR workflow
- Standard real-time PCR conditions
- Room temperature setup
- Compatible with probe detection
- Streamlined processing

Keywords: RT-PCR, probe-based qPCR, gene expression analysis, RNA quantification