HotStart PCR 2x Master Mix in Green

A pre-formulated PCR master mix containing chemically modified HotStart Taq DNA polymerase with integrated green tracking dye for direct gel loading capability.

Technical Specifications:
- Format: 2X concentrated
- Enzyme: HotStart Taq DNA Polymerase
- Activation: 3-5 minutes at 95°C
- Amplification range: Up to 7 kb
- Product ends: Blunt and 3' A-overhang
- Visual indicator: Green dye included

Master Mix Properties:
- Chemical hot-start activation
- Pre-optimized buffer system
- Direct gel loading capability
- Room temperature setup
- Standard PCR cycling conditions

Research Applications:
- Standard PCR protocols
- High-throughput PCR
- Colony PCR screening
- PCR cloning
- Template amplification
- Fragment analysis

Protocol Features:
- Minimized non-specific amplification
- Reduced primer-dimer formation
- Visual reaction monitoring
- Simplified reaction setup
- Template and primer addition only

Keywords: hot start PCR, ready-to-use master mix, PCR optimization, molecular biology reagents