High Fidelity PCR Master Mix HFL, 2X

A pre-formulated 2X PCR master mix designed for high-fidelity DNA amplification and long-range PCR applications. Contains modified thermostable DNA polymerases with proofreading capability in an optimized reaction buffer.

Technical Specifications:
- Format: 2X concentrated master mix
- End modification: Blunt-ended products
- Tracking dyes included: Blue and yellow indicators
- Direct gel loading capability
- Pre-optimized buffer composition

Master Mix Components:
- Modified thermostable DNA polymerases
- PCR reaction buffer
- dNTPs
- Gel tracking dyes
- Stabilizers and enhancers

Research Applications:
- Long-range PCR amplification
- High-fidelity DNA synthesis
- Molecular cloning projects
- Promoter analysis studies
- RACE protocols
- DNA sequencing templates
- GC-rich template amplification

Protocol Features:
- Simplified reaction setup
- Requires only primers and template
- Visual monitoring of electrophoresis
- Compatible with standard thermal cyclers
- Room temperature reaction assembly

Keywords: high-fidelity PCR, long-range PCR, molecular biology reagents, DNA amplification, proofreading PCR mix