dNTP set
dNTP Set
A set of purified deoxynucleotide triphosphates (dATP, dCTP, dGTP, dTTP) for nucleic acid synthesis applications, tested for nuclease and phosphatase contamination.
Technical Specifications:
- Chemical purity: ≥99% triphosphate by HPLC
- Concentration: 100mM each nucleotide
- Volume: 400µl each nucleotide
- Amount: 40µmol each nucleotide
- Formulation: In sterile, double-distilled water
Quality Control Testing:
- Nuclease-free certification
- Phosphatase-free certification
- Sanger sequencing verification
- Klenow fragment activity testing
- Thermostable polymerase compatibility
- Spectrophotometric concentration verification
Research Applications:
- Standard PCR protocols
- RT-PCR procedures
- Long-range PCR
- Real-time PCR
- Reverse transcription
- Site-directed mutagenesis
- DNA sequencing
- Primer extension
Storage and Handling:
- Store at -20°C
- Dilute with sterile, double-distilled water
- Avoid repeated freeze-thaw cycles
Keywords: deoxynucleotide triphosphates, PCR