Column-Pure™ Plasmid Mini-Prep Kit

A silica membrane-based spin column system designed for rapid isolation and purification of plasmid DNA from bacterial cultures. The kit provides reagents and columns for obtaining high-quality plasmid DNA suitable for molecular biology applications.

Technical Specifications:
- DNA purity: 1.8-2.0 OD260/280 ratio
- DNA conformation: Primarily supercoiled
- Column type: Silica membrane
- Processing capacity: Mini-prep scale
- Elution volume: Adjustable

Kit Components:
- Solution I (Resuspension Buffer)
- Solution II (Lysis Buffer)
- Solution III (Neutralization Buffer)
- Wash Solution
- Elution Buffer
- Spin Columns
- RNase A
- Detailed protocol manual

Research Applications:
- DNA sequencing templates
- Restriction enzyme analysis
- Bacterial transformation
- Cloning procedures
- PCR template preparation
- Standard molecular biology protocols

Protocol Features:
- Rapid isolation procedure
- No organic extraction required
- Room temperature processing
- Standard microcentrifuge compatible
- Reproducible DNA recovery

Keywords: plasmid isolation, DNA purification, mini-prep kit, molecular biology reagents, bacterial plasmid prep