Column-Pure™ PCR Clean-Up Kit

A silica membrane-based purification system designed for the isolation and cleanup of PCR products. This kit enables removal of reaction components while maintaining DNA integrity for downstream applications.

Technical Specifications:
- Fragment size range: 100 bp to 10 kb
- Silica membrane technology
- Adjustable elution volume for concentration
- Compatible with standard microcentrifuges
- Removes primers, enzymes, salts, and dNTPs

Kit Components:
- Binding Buffer
- Wash Buffer
- Elution Buffer
- Spin Columns
- Detailed protocol manual

Purification Capabilities:
- PCR product recovery
- Primer removal
- Enzyme elimination
- Salt removal
- Buffer exchange
- Sample concentration

Research Applications:
- Sequencing preparation
- Cloning procedures
- Restriction digestion
- Labeling reactions
- Microarray analysis
- Next-generation sequencing

Protocol Features:
- Single-spin process
- Room temperature procedure
- Standard laboratory equipment compatible
- Flexible elution volumes

Keywords: PCR purification, DNA cleanup, molecular biology reagents, PCR product isolation