cDNA Synthesis: EasyScript Plus™ Reverse Transcriptase
EasyScript Plus™ Reverse Transcriptase
A recombinant reverse transcriptase engineered for cDNA synthesis at elevated temperatures, designed to handle RNA templates with complex secondary structures and high GC content.
Technical Specifications:
- Working temperature: 50-55°C
- Template capacity: Up to 12 kb
- Activity: RNA-dependent DNA polymerase
- Format: Ready-to-use enzyme
- Engineered for complex templates
Enzyme Properties:
- High-temperature activity
- Secondary structure handling
- GC-rich template compatibility
- Full-length cDNA synthesis
- Thermostable reverse transcription
Research Applications:
- RT-PCR
- Real-time RT-PCR
- cDNA library construction
- Serial Analysis of Gene Expression (SAGE)
- Rapid Amplification of cDNA Ends (RACE)
- Gene expression analysis
- RNA sequence analysis
Protocol Features:
- Standard reaction conditions
- Compatible with common primers
- Room temperature setup
- Flexible buffer conditions
- Standard laboratory equipment compatible
Keywords: reverse transcriptase, cDNA synthesis, RNA analysis, molecular biology reagents