cDNA Synthesis: 5X All-in-One RT Plus MasterMix
5X All-in-One RT Plus MasterMix
A concentrated master mix designed for first-strand cDNA synthesis, featuring EasyScript Plus™ Reverse Transcriptase in a 5X formulation for reaction volume flexibility.
Master Mix Components:
- EasyScript™ Plus Reverse Transcriptase
- Ribonuclease inhibitor
- cDNA synthesis enhancers
- dNTPs
- Oligo(dT) and random primers
- Temperature-sensitive DNase
Technical Features:
- 5X concentration format
- Combined oligo(dT) and random primer system
- Genomic DNA removal capability
- Single-tube reaction setup
- Compatible with variable RNA input amounts
Research Applications:
- First-strand cDNA synthesis
- Gene expression analysis
- RT-qPCR experiments
- RNA analysis protocols
- Transcriptome studies
Protocol Benefits:
- Single-tube reaction format
- Streamlined pipetting steps
- Flexible reaction volumes
- Compatible with standard thermal cyclers
- Room temperature setup capability
Keywords: cDNA synthesis, reverse transcription, RT-PCR reagents, gene expression analysis, RNA analysis tools, molecular biology reagents