Bacterial Medium: Terrific Broth

A pre-formulated rich growth medium powder optimized for high-density bacterial cultures and enhanced protein expression. Designed for improved yield in molecular biology applications.

Technical Specifications:
- Format: Powder
- Package size: 500g
- Standard concentration: 47.6g/L
- pH: 7.2 ± 0.2 at 25°C
- Autoclave compatible

Composition per Liter:
- Tryptone: 12g
- Yeast Extract: 24g
- K2HPO4: 9.4g
- KH2PO4: 2.2g

Preparation Protocol:
1. Weigh 47.6g powder per liter
2. Add appropriate volume of water
3. Mix until dissolved
4. Autoclave at 121°C for 15 minutes
5. Cool to room temperature before use

Research Applications:
- High-density bacterial culture
- Protein expression
- Plasmid production
- Recombinant protein synthesis
- Bacterial fermentation
- Molecular biology studies

Storage Requirements:
- Store powder at room temperature
- Keep container tightly sealed
- Protect from moisture
- Prepared medium stable at 4°C

Keywords: bacterial growth medium, Terrific Broth, culture media, molecular biology reagents