Bacterial Medium: LB Broth

A pre-formulated Luria-Bertani (LB) broth powder designed for bacterial culture applications in molecular biology research. Optimized for consistent bacterial growth and protein expression studies.

Technical Specifications:
- Format: Powder
- Package size: 500g
- Standard concentration: 20g/L
- pH: 7.0 ± 0.2 at 25°C
- Autoclave compatible

Composition per Liter:
- Tryptone: 10g
- Yeast Extract: 5g
- Sodium Chloride: 10g

Preparation Protocol:
1. Weigh 20g powder per liter
2. Add appropriate volume of water
3. Mix until dissolved
4. Autoclave at 121°C for 15 minutes
5. Cool to room temperature before use

Research Applications:
- Bacterial culture
- Protein expression
- Plasmid preparation
- Competent cell preparation
- Molecular biology studies
- Microbiology research

Storage Requirements:
- Store powder at room temperature
- Keep container tightly sealed
- Protect from moisture
- Prepared medium stable at 4°C

Keywords: bacterial growth medium, LB broth, culture media, molecular biology reagents