Bacterial Medium: 2xYT Broth

A pre-formulated rich growth medium powder designed for high-yield bacterial culture applications. Features increased nutrient concentration for enhanced bacterial growth and protein expression.

Technical Specifications:
- Format: Powder
- Package size: 500g
- Standard concentration: 31g/L
- pH: 7.0 ± 0.2 at 25°C
- Autoclave compatible

Composition per Liter:
- Tryptone: 16g
- Yeast Extract: 10g
- Sodium Chloride: 5g

Preparation Protocol:
1. Weigh 31g powder per liter
2. Add appropriate volume of water
3. Mix until dissolved
4. Autoclave at 121°C for 15 minutes
5. Cool to room temperature before use

Research Applications:
- High-density bacterial culture
- Protein expression systems
- Phage production
- Plasmid preparation
- Bacterial transformation
- Molecular biology studies

Storage Requirements:
- Store powder at room temperature
- Keep container tightly sealed
- Protect from moisture
- Prepared medium stable at 4°C

Keywords: bacterial growth medium, 2xYT broth, culture media, molecular biology reagents