The AccuRuler Prestained Protein Ladder Plus contains 12 prestained proteins, covering a wide range of molecular weights from 10 to 245kDa.  It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins.  The ladder is supplied in protein gel loading buffer and is ready-to-use.  No need to heat, dilute, or add reducing agent before loading.The Pico Ultra ECL Western Blotting Detection Kit contains 100ml of Solution A and 100 ml of Solution B.  Mix Solutions A and B in a 1:1 ratio right before the application.  The mixture is then ready for detection of any immobilized specific antigens in chemiluminescent Western blots through horseradish peroxidase (HRP) labeled antibodies.  The kit is designed for a high quality and long lasting glow.