AccuRuler Broad Prestained Protein Ladder

A pre-stained protein molecular weight marker containing 12 proteins with expanded range from 10 to 310 kDa. Designed for protein separation monitoring and molecular weight determination in electrophoresis and Western blot applications.

Technical Specifications:
- Size range: 10-310 kDa
- Number of bands: 12
- Volume: 2 x 250 µl
- Recommended loading: 3-5 µl
- Storage: -20°C

Buffer Composition:
- 20 mM Trisphosphate (pH 7.5 at 25°C)
- 2% SDS
- 1 mM reducing reagent
- 3.6 M Urea
- Protein concentration: 0.1-0.4 mg/ml each
- Includes stabilizers

Research Applications:
- Protein size determination
- PAGE protein separation
- Western blot transfer monitoring
- Molecular weight estimation
- Protein migration tracking

Protocol Features:
- Ready-to-use format
- No heating required
- No dilution needed
- No additional reducing agents required
- Room temperature handling

Quality Control:
- Verified by PAGE
- Western blot tested
- Transfer efficiency validated

Keywords: protein ladder, broad range marker, Western blot standard, PAGE marker