1Kb DNA Ladder, 5 tubes
1Kb DNA Ladder™ (5x 1000µl)
The 1Kb DNA Ladder™ is a precision molecular weight marker containing 11 DNA fragments ranging from 0.5-10.0Kb. Generated through specific restriction digestion, this ladder provides accurate size determination for larger DNA fragments.
Key Features:
- 11 distinct fragments (0.5-10.0Kb range)
- Enhanced reference bands
- Ready-to-use format
- Room temperature stable
- 5x 1000µl volume
Technical Specifications:
- Fragment range: 0.5-10.0Kb
- Concentration: 500ng/6µl
- Minimum yield: 30ng per band (6µl loading)
- Storage: -20°C
- Pre-mixed with loading buffer
Buffer Composition:
- 10mM Tris-HCl
- 1mM EDTA (pH 8.0)
- 0.02% Bromophenol blue
- 0.02% Xylene cyanol
- 5% Glycerol
Research Applications:
- Large fragment analysis
- Restriction digest verification
- Molecular cloning
- Genomic DNA analysis
- Plasmid linearization check
Usage Guidelines:
- Recommended loading: 6µl minimum
- Adjust volume based on well size
- Load directly without additional buffer
- Allow for complete well visualization
- Optimized for standard gel concentrations
Keywords: DNA ladder, kilobase marker, DNA fragment analysis, agarose gel electrophoresis, large fragment standard, molecular biology reagents